Mitomycin, one of the mitosane antibiotics, is a alkylating agent in common use as a anticancer drug, which is isolated from Streptomyces caespitosus culture media. Mitomycin interferes with the template activities of DNA by cross linking to the DNA double helix and inhibites nucleic acid synthesis
The authors have demonstrated the effect of mitomycin on the liver of albino mice morphologically, observing the cellular change and accumulation of fat in the hepatic parenchymal cells.
A total of 52 healthy albino mice of ICR strain (the weight 20g) were divided into two groups; the control and the mitomycin treated groups.
All, experimental animals were given food and water ad libitum. The animals of the mitomycin treated groups were administered 4mg per kg of, body weight of ´mitomycin diluted in the water for° injection. The animals of the control groups were administered only water for injection. The animals of the mitomycin treated groups were sacrified at intervals of. 6, 12, 24 and 36 hours after administration of mitomycin.
Histological preparations were prepared by way of hematoxylin and eosin stain for the cellular changes and oil red 0; stain for fat accumlation in the mouse liver.
The results are obtained as follow:
1. The cellular swelling associated with vesicular formation are observed in the hepatic parenchymal cells of the central and intermediate zones of hepatic lobule at 12 and 24 hours after administration of mitomycin.
2. Fat deposits in the hepatic parenchymal cells of the central zones of hepatic lobule; are observed at 24 hours after administration of mitomycin.
It is suggested that mitomycin causes cellular swelling accompanied with the vesicular formation and fat accumulation in the parenchymal cells of the liver in mice.
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